Proteins are the effectors of all activities within cells. The expression of certain proteins change upon cell stimulation or stress, or when cells differentiate or turn into a disease state. The measurement of protein expression levels as well as the characterization of their post-translational modifications gives information about their cellular state. The goal of quantitative proteomics is to evaluate the relative expression of proteins between two cellular samples being compared.
Mass spectrometry (MS) is used for the quantification and identification of molecules. For the accurate quantification of proteins, the stable isotope labelling of amino acids in cell culture (SILAC) is a versatile tool. For this purpose, reference molecules differing from the sample molecule with regards to isotopic labelling (2H, 13C, 15N) are necessary. Silantes offers methods for SILAC (Stable Isotope Labelling with Amino acids in Cell culture) and SILAM (Stable Isotope Metabolic Labelling in Mammals) to produce these reference molecules.
With our products that have an isotopical enrichment of >98%, we guarantee successful research projects with precise results.
In-vitro SILAC Proteomics Media and KitsSilac media - Silantes offers all components necessary for a SILAC experiment. |
In-vitro SILAC CTAP with DAPSilantes offers all components necessary for cell type specific labelling using amino acid precursors (SILAC CTAP). |
In-vivo Mouse SILAC ProteomicsIsotopically labelled animal diets as well as metabolically labelled animal tissues of mice, including
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In-vivo Mouse SILAM Proteomics/MetabolomicsIsotopically labelled animal diets as well as metabolically labelled animal tissues of mice, including
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In-vivo Fly SILAC ProteomicsIsotopically labelled diets for the cultivation of flies for the SILAC method, including
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In-vivo Fly SILAM Proteomics/MetabolomicsIsotopically labelled diets for the cultivation of flies for the SILAM method, including
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Modified Nucleosides and NucleotidesSilantes offers nucleoside and nucleotides modifications as internal standard for quantitative mass spectrometry. |
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