Print

Products for Mass Spectrometry

 

Proteins are the effectors of all activities within cells. The expression of certain proteins change upon cell stimulation or stress, or when cells differentiate or turn into a disease state. The measurement of protein expression levels as well as the  characterization of their post-translational modifications gives information about their cellular state. The goal of quantitative proteomics is to evaluate the relative expression of proteins between two cellular samples being compared.

Mass spectrometry (MS) is used for the quantification and identification of molecules.  For the accurate quantification of proteins, the stable isotope labelling of amino acids in cell culture (SILAC) is a versatile tool. For this purpose, reference molecules differing from the sample molecule with regards to isotopic labelling (2H, 13C, 15N) are necessary. Silantes offers methods for SILAC (Stable Isotope Labelling with Amino acids in Cell culture) and SILAM (Stable Isotope Metabolic Labelling in Mammals) to produce these reference molecules.

With our products that have an isotopical enrichment of >98%, we guarantee successful research projects with precise results.

 


In-vitro SILAC Proteomics Media and Kits

SILAC_media_and_Kits_final

Silac media - Silantes offers all components necessary for a SILAC experiment.
Components can be purchased in the webshop as single components or as a compound kit.

In-vitro SILAC CTAP with DAP

SILAC_media_and_Kits_final

Silantes offers all components necessary for cell type specific labelling using amino acid precursors (SILAC CTAP).

In-vivo Mouse SILAC Proteomics

Products_for_in-vivo_SILAC_final

Isotopically labelled animal diets as well as metabolically labelled animal tissues of mice, including

  • Lys(0)-labelled mouse diets,
  • Lys(8)-labelled mouse diets,
  • Lys(6)-labelled mouse diets,
  • Lys(0)-labelled mouse tissues and
  • Lys(6)-labelled mouse tissues.

In-vivo Mouse SILAM Proteomics/Metabolomics

Products_for_in-vivo_SILAM_final

Isotopically labelled animal diets as well as metabolically labelled animal tissues of mice, including

  • 14N-labelled mouse diets,
  • 15N-labelled mouse diets,
  • 14N-labelled mouse tissues and
  • 15N-labelled mouse tissues,
  • Partially 13C-labelled Ralstonia mouse diets,
  • Uniformly 13C-labelled Spirulina mouse diets,

In-vivo Fly SILAC Proteomics

Products_for_in-vivo_Fly_SILAC

Isotopically labelled diets for the cultivation of flies for the SILAC method, including

  • Lys(0)-labelled fly diets,
  • Lys(4)-labelled fly diets,
  • Lys(6)-labelled fly diets,
  • Lys(8)-labelled fly diets.

 

In-vivo Fly SILAM Proteomics/Metabolomics

Products_for_in-vivo_Fly_SILAM

Isotopically labelled diets for the cultivation of flies for the SILAM method, including

  • 14N-labelled fly diets,
  • 15N-labelled fly diets.

Modified Nucleosides and Nucleotides

Modified_Nucleosides_Nucleotides_as_internal_standards

Silantes offers nucleoside and nucleotides modifications as internal standard for quantitative mass spectrometry.