Product description: Silantes Lysine(6) lyophilized mouse liver, in Urea buffer

Content:

- 1 x SILAM Mouse Liver Tissue

- Amount: 100 µg of buffered protein

- This product is in powder form (lyophilized from the wet tissue)

- Isotopic enrichment for ¹³C-Lysine > 97 atom %

Specifications:

- Strain: C57BL/6

- ¹³C-Lys-labeled for at least 2 generations (F2)

Buffer information: (for Urea)

- 6 M Urea

- 2 M Thiourea

- 10 mM HEPES pH 7.4

Silantes GmbH provides metabolically labeled tissues of mice fed with labeled diets produced and developed in- house.

References:

- Bock, T., Türk, C., Aravamudhan, S. et al. PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B. Nat Commun 12, 4900 (2021). https://doi.org/10.1038/s41467-021-25185-3

- Rolfs, Z., Frey, B.L., Shi, X. et al. An atlas of protein turnover rates in mouse tissues. Nat Commun 12, 6778 (2021). https://doi.org/10.1038/s41467-021-26842-3

- Meschkat, M., Steyer, A.M., Weil, MT. et al. White matter integrity in mice requires continuous myelin synthesis at the inner tongue. Nat Commun 13, 1163 (2022). https://doi.org/10.1038/s41467-022-28720-y

- Meschkat, M., Steyer, A.M., Weil, MT. et al. White matter integrity in mice requires continuous myelin synthesis at the inner tongue. Nat Commun 13, 1163 (2022). https://doi.org/10.1038/s41467-022-28720-y

- Rinschen, M. M., Gödel, M., Grahammer, F., Zschiedrich, S., Helmstädter, M., Kretz, O., Zarei, M., Braun, D. A., Dittrich, S., Pahmeyer, C., Schroder, P., Teetzen, C., Gee, H., Daouk, G., Pohl, M., Kuhn, E., Schermer, B., Küttner, V., Boerries, M., … Huber, T. B. (2018). A multi- layered quantitative in 1vivo expression atlas of the podocyte unravels kidney disease candidate genes. Cell Reports, 23 (8), 2495–2508. https://doi.org/10.1016/j.celrep.2018.04.059

- Arike, L., Seiman, A., van der Post, S., Rodriguez Piñeiro, A. M., Ermund, A., Schütte, A., Bäckhed, F., Johansson, M. E. V., &1 Hansson, G. C. (2020). Protein turnover in epithelial cells and mucus along the gastrointestinal tract is coordinated by the spatial location and microbiota. Cell Reports, 30(4). https://doi.org/10.1016/j.celrep.2019.12.068

- Andrews, B., Murphy, A. E., Stofella, M., Maslen, S., Almeida-Souza, L., Skehel, J. M., Skene, N. G., Sobott, F., &1 Frank, R. A. W. (2022). Multidimensional Dynamics of the proteome in the neurodegenerative and aging mammalian brain. Molecular &1amp1 Cellular Proteomics, 21(2), 100192. https://doi.org/10.1016/j.mcpro.2021.100192

- Kallabis S, Abraham L, Müller S, Dzialas V, Türk C, Wiederstein JL, Bock T, Nolte H, Nogara L, Blaauw B, Braun T, Krüger M. High-throughput proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers. Skelet Muscle. 2020 Mar 23110(1):7. doi: 10.1186/s13395-020-00226-5. PMID: 322935361 PMCID: PMC7087369.