Product description: Silantes SILAC RPMI FBS Kit
Content of the kit:
- 1 x SILAC RPMI (Dulbecco's Modified Eagle Medium) without L-Glutamine, L-Arginine and L-Lysine, amount: 500 mL
- 1 x L-Glutamine, isotopic labelling: unlabelled, amount: 5 mL , in solution, concentration: c=0.2 mol/L, molecular weight = 146.1 g/mol, chemical formula: C5H10N2O3, chemical purity > 95 %
- 1 x SILAC FBS (Fetal Bovine Serum, dialyzed), amount: 50 mL
All products are sterile.
Dry ice shipment required.
The SILAC-procedure in short:
Cells are grown in culture media that are identical with exception of amino acids. The “light” culture contains all the amino acids in unlabelled form and the “heavy” culture contains one or two amino acids in labelled form. The labelled amino acids are usually L-Lysine labelled with 2H (D4),13C or 13C15N and/or arginine labelled with 13C or 13C15N. The labelled amino acids increase the molecular weight allowing identification and quantification of the proteins/peptides stemming from culture light and heavy, respectively.