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Rich Growth Media

Rich Growth Media

 

Structural studies on proteins by nuclear magnetic resonance (NMR) require labelling of these molecules with stable isotopes. Organisms such as bacteria and yeast are used to express these proteins. Silantes offers a wide range of stable isotope-labelled

to express stable isotope-labelled proteins.

Silantes OD media are rich growth media for the expression of stable isotope labelled protein. The media are available in all combinations of the stable isotopes 2H, 13C and 15N. They are formulated for increasing cell densities (optical densities measured at 600nm) containing hydrolysates with increasing concentrations.

 

High Isotopic Enrichment

The media are available in all combinations of the isotopic labels 2H, 13C, 15N with an isotopic enrichment of > 98 %.

 

Available in Multiple Variations

The media are available

  • for E.coli and yeast,
  • as powder and as solution,
  • in different optical densities OD1, OD2, OD5 and OD10,
    • Silantes OD1 media reach a cell density comparable to 0,2% glucose-M9 media.
    • Silantes OD2 media reach a cell density comparable to 0,4% glucose-M9 media. Silantes OD2 media are commonly used if inducing the cells at OD0,6-0,9 followed by a ~4h incubation. The heterologous protein expression performance is similar as with LB medium.
    • Silantes OD4-, OD5- and OD10 (concentrate)-media are available on request.
  • in quantities of 100 mL, 200 mL, 500 mL and 1 L (solution) or 1 g, 10 g, 100 g (powder).

 

Excellent Performance, expecially with 2H (D) - Labelling

Figure 1 shows the growth curve of E.coli in “2H-labelled Silantes OD2 Rich Growth Media for E.coli” and in 1 % “unlabeled Silantes Powder Media for E.coli” in D2O. The figure shows that both media have the same excellent performance and exceed OD 2.5 in less than 24 hours.

Figure 1: Growth of E.coli 2H-labelled Silantes media solution and in Silantes powder media in D2O

 

 

No Glucose Needed

The Silantes Rich Growth Media are prepared from uniformly labelled bacterial cell hydrolysate of Ralstonia eutropha containing carbon as energy source. Therefore no additional 13C-glucose is necessary to produce 13C-labelled proteins.

 

 

Cost-Effective

Figure 1 and figure 2 show SDS-PAGEs as an example of protein expressions using M9 media and Silantes Rich Growth media for ubiquitin and RNA polymerase α-subunit. The figures show that the yield is higher when Silantes Rich Growth Media is used.

Figure 2: SDS-Page Ubiquitin

 

Figure 3: SDS-Page RNA Polym. a-subunit

 

 

Consistently High Quality

The Silantes Rich Growth Media are checked for reproducible fermentation results. Moreover, each media batch is adjusted to guarantee consistent cell densities.

 

 

Ready-to-Use

The Silantes Rich Growth Media are sterile and Ready-to-Use for fermentations.

 

 

Request here for a free Silantes Rich Growth Media sample!

 


Related topics: Silantes stable isotope labelled chemicals such as D2O, 13C-glucose and 15NH4Cl