Structural studies on proteins by nuclear magnetic resonance (NMR) require labelling of these molecules with stable isotopes. Organisms such as bacteria and yeast are used to express these proteins. Silantes offers a wide range of stable isotope-labelled
to express stable isotope-labelled proteins.
Silantes OD media are rich growth media for the expression of stable isotope labelled protein. The media are available in all combinations of the stable isotopes 2H, 13C and 15N. They are formulated for increasing cell densities (optical densities measured at 600nm) containing hydrolysates with increasing concentrations.
High Isotopic Enrichment
The media are available in all combinations of the isotopic labels 2H, 13C, 15N with an isotopic enrichment of > 98 %.
Available in Multiple Variations
The media are available
Excellent Performance, expecially with 2H (D) - Labelling
Figure 1 shows the growth curve of E.coli in “2H-labelled Silantes OD2 Rich Growth Media for E.coli” and in 1 % “unlabeled Silantes Powder Media for E.coli” in D2O. The figure shows that both media have the same excellent performance and exceed OD 2.5 in less than 24 hours.
No Glucose Needed
The Silantes Rich Growth Media are prepared from uniformly labelled bacterial cell hydrolysate of Ralstonia eutropha containing carbon as energy source. Therefore no additional 13C-glucose is necessary to produce 13C-labelled proteins.
Figure 1 and figure 2 show SDS-PAGEs as an example of protein expressions using M9 media and Silantes Rich Growth media for ubiquitin and RNA polymerase α-subunit. The figures show that the yield is higher when Silantes Rich Growth Media is used.
Consistently High Quality
The Silantes Rich Growth Media are checked for reproducible fermentation results. Moreover, each media batch is adjusted to guarantee consistent cell densities.
The Silantes Rich Growth Media are sterile and Ready-to-Use for fermentations.