Print

Phosphoramidites

Phosphoramidites

 

DEOXYRIBOPHOSPHORAMIDITES

These amidites are uniformly labeled (in the nucleotide part) and supplied as 13C- and 15N- labeled compounds. They are chemically synthesized from deoxynucleosides obtained from 13C- or 15N- labeled DNA or RNA, respectively.

This organic-biochemical approach permits us to offer the deoxyphosphoramidites at very competitive prices.

 

RIBOPHOSPHORAMIDITES

These amidites are available site-specifically labeled in a variety of patterns of 2H, 13C and 15N labels.

 

SUPPLY

The amidites are supplied in 10 mg and 50 mg quantities and delivered in vials suitable for chemical synthesis.

 

QUALITY

The isotopic enrichment of the amidites is >98 % with a chemical purity of >95 %. This is confirmed by mass spectrometric analysis supplied with each nucleotide and verified by 31P-NMR (see below). The figure verifies the absence of phosphonate at 150ppm as a well-known contamination at the synthesis of deoxyribophosphoramidites.

 

Phosphoramidite_31P_NMR_Spectra_with_absence

 


Silantes also offers a custom synthesis service for deoxy- and ribophosphoramidites.

Further information on the Silantes phosphoramidite oligosynthesis services.



Ribo-Phosphoramidites

Phosphoramidites_allgemein

Ribo-Phosphoramidites (rA, rC, rG, rU) in multiple variations of the stable isotopes 2H, 13C and 15N (partially labeled) for oligosynthesis.

Deoxyribo-Phosphoramidites

Phosphoramidites_allgemein

Deoxyribo-Phosphoramidites (dA, dC, dG, dT) in all variations of the stable isotopes 2H, 13C and 15N (uniformly labeled) for oligosynthesis.

Get the deoxyribo-Phosphoramidite-Sets here!