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Phosphoramidites

Phosphoramidites

 

Structural studies on large DNA and RNA molecules by nuclear magnetic resonance (NMR) require labelling of these molecules with stable isotopes.

Silantes offers a wide range of stable isotope labelled

in many combinations of the stable isotopes 2H, 13C and 15N for the chemical synthesis of oligonucleotides (click here for information about the Silantes chemical synthesis service). Ths isotopic purity is >98 %. The phosphoramidites are available in 10 mg or 50 mg quantities.

 


Silantes also offers stable isotope labelled nucleosides and nucleotides as well as a custom enzymatic oligo-synthesis service.


 

DEOXYRIBOPHOSPHORAMIDITES

These amidites are uniformly labeled (in the nucleotide part) and supplied as 13C- and 15N- labeled compounds. They are chemically synthesized from deoxynucleosides obtained from 13C- or 15N- labeled DNA or RNA, respectively.

This organic-biochemical approach permits us to offer the deoxyphosphoramidites at very competitive prices.

 

RIBOPHOSPHORAMIDITES

These amidites are available site-specifically labeled in a variety of patterns of 2H, 13C and 15N labels.

 

SUPPLY

The amidites are supplied in 10 mg and 50 mg quantities and delivered in vials suitable for chemical synthesis.

 

QUALITY

The isotopic enrichment of the amidites is >98 % with a chemical purity of >95 %. This is confirmed by mass spectrometric analysis supplied with each nucleotide and verified by 31P-NMR (see below). The figure verifies the absence of phosphonate at 150ppm as a well-known contamination at the synthesis of deoxyribophosphoramidites.

 

Phosphoramidite_31P_NMR_Spectra_with_absence