Silantes offers high quality stable isotope labelled
for monitoring the performance of NMR spectrometers and for testing new pulse-sequences.
High Isotopic Enrichment > 98 %
The isotopic enrichment of the NMR standards is verified by NMR analysis. Figure 1 shows a 2D-NMR spectrum with two superimposed ubiquitin samples: Triple-labelled ubiquitin (2H, 13C, 15N) in blue and double-labelled ubiquitin (13C, 15N) in red. Click here to find the 2D-NMR spectra for other labellings.
Figure 1: 2D NMR spectra for 2H13C15N- (blue) and 13C15N- (red) labelled ubiquitin. Data of this figure were kindly provided by Dr. Richter in Prof. Dr. Harald Schwalbe Group, Goethe University Frankfurt.
Hich Chemical Purity > 95 %
The chemical purity of the NMR standards is verified by electrophoresis for ubiquitin (see figure 2) and ion-exchange chromatography for RNA standards (see figure 3).
Figure 2: SDS-PAGE for ubiquitin
Figure 3: Ion exchange elution profile of a 14mer RNA fragment (5' - pppGGCACUUCGGUGCC - 3')
Our NMR standards are available in both solid powder form and as concentrated solution (550 µL) prepackaged in a ready-to-use 5 mm NMR tube.