RNA modifications can be found in a wide range of organisms and in almost all RNA species, including tRNA, mRNA and small non-coding RNAs. These modifications are of great interest in scientific research on cellular functions related to RNA recognition, stability, and delivery. In addition, RNA based gene regulation was shown to be impacted by RNA modifications in health and disease. The determination of the stoichiometry of an RNA modification is critical since it can vary depending on the cellular stimulus like nutrition availability, stress, signal transduction, etc.

Stable isotope labelled nucleosides and nucleotides for quantification of RNA modifications by mass spectrometry

The analysis, detection, and quantification of RNA modifications by high throughput methods is currently a very active research field in both academia and industry. For their quantification with mass spectrometry, stable isotope labelled modified nucleosides/nucleotides are required, which are of limited commercial availability.

Silantes has developed stable isotope labelled methylation nucleobases that can be used as internal standards for quantification by mass spectrometry.

In this webshop you can find a range of naturally occurring modified RNA nucleosides with stable isotope labelling patterns. The modified nucleobases are enhanced in mass by deuterium (²H), stable carbon isotope (¹³C) or stable nitrogen isotope (¹⁵N).

The modification you desire with the mass shift you require

In addition to the product examples shown in this webshop, Silantes can synthesize individually required molecules.

Customized modifications

Silantes can provide all bases (adenine, guanine, cytosine, uracil) either as nucleoside, mono-, di-, or triphosphate and with all common modifications (e.g., methylations, pseudouridine, etc.).

Customized labelling patterns

In addition to the labeling patterns and the associated mass shifts indicated in this webshop, Silantes can also realize an individually requested labelling pattern site-specifically in base, ribose, or modification.

To request a quotation for the products listed or for a custom synthesis, please contact our customer service.