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Products for Proteomics

 

Proteins are the effectors of all activities within cells. The expression of certain proteins change upon cell stimulation or stress, or when cells differentiate or turn into a disease state. The measurement of protein expression levels as well as the  characterization of their post-translational modifications gives information about their cellular state. The goal of quantitative proteomics is to evaluate the relative expression of proteins between two cellular samples being compared.

Mass spectrometry (MS) is used for the quantification and identification of molecules.  For the accurate quantification of proteins, the stable isotope labelling of amino acids in cell culture (SILAC) is a versatile tool. For this purpose, reference molecules differing from the sample molecule with regards to isotopic labelling (2H, 13C, 15N) are necessary. Silantes offers methods for SILAC (Stable Isotope Labelling with Amino acids in Cell culture) and SILAM (Stable Isotope Metabolic Labelling in Mammals) to produce these reference molecules.

With our products that have an isotopical enrichment of >98%, we guarantee successful research projects with precise results.

 


In-vitro SILAC Media and Kits

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Silantes offers all components necessary for a SILAC experiment, including

  • unlabelled and stable isotope labelled L-lysine,
  • unlabelled and stable isotope labelled L-arginine,
  • FBS,
  • DMEM without L-lysine and L-arginine and
  • RPMI without L-lysine and L-arginine.

These components can be purchased in the webshop either as single components or as a compound kit.

Products for in-vivo SILAC

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Isotopically labelled animal diets as well as metabolically labelled animal tissues of mice, including

  • Lys(0)-labelled mouse diets,
  • Lys(6)-labelled mouse diets,
  • Lys(0)-labelled mouse tissues and
  • Lys(6)-labelled mouse tissues.

Products for in-vivo SILAM

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Isotopically labelled animal diets as well as metabolically labelled animal tissues of mice, including

  • 14N-labelled mouse diets,
  • 15N-labelled mouse diets,
  • 14N-labelled mouse tissues and
  • 15N-labelled mouse tissues.