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Nucleosides/-tides

Nucleosides/-tides

 

Structural studies on large DNA and RNA molecules by nuclear magnetic resonance (NMR) require labelling of these molecules with stable isotopes.

Silantes offers a wide range of stable isotope labelled

in any combination of the stable isotopes 2H, 13C and 15N for enzymatic synthesis of oligonucleotides (click here for information about the Silantes enzymatic synthesis service). The isotopic purity is >98 %. The nucleotides are available in 10 mg or 50 mg quantities as solution in lithium salt. Modifications are available on request.

 

 


Silantes also offers stable isotope labelled phosphoramidites as well as a custom chemical oligo-synthesis service.


 

 

The Silantes technology of labelling nucleosides and nucleotides

Silantes stable isotope labelled nucleotides are prepared from bacterial DNA and RNA. The strain is a chemolithoautotrophic organism which grows on H2, O2 and CO2. The extracted DNA or RNA is enzymatically hydrolyzed. The isolated 5'-NMPs are enzymatically phosphorylated to 5'-NDPs and 5'-NTPs and purified by IC and RP-HPLC.

This technology for in vivo enrichment of stable isotopes enables us to offer Silantes nucleosides and nucleotides at very competitive prices.

 

High quality, biological competence and ready-to-use

We guarantee an isotopic enrichment of >98 % with a chemical purity of >95 % determined by HPLC. Figure 1 shows an example of the HPLC elution profile of CTP.

 

Example_Quality_Analysis_of_CTP_by_RP-HPLC

Figure 1: HPLC elution profile of CTP

 

The biological competence of the nucleotides is validated by in vitro synthesis of DNA or RNA, respectively.

Figure 2 shows the yield of an oligonucleotide synthesis using 15N-labelled rNTPs (left) and 13C15N-labelled dNTPs (right) in different salts.

 

Figure 2: Kinetics of the synthesis of ribooligonucleotides (left) and deoxyribooligonucleotides (right) in Li-, Na- and NH4-salt.

 

The analysis shows that the performance as well as the yield of an oligo synthesis does not depend on the salting of the NTPs. Silantes nucleotides are usually supplied in lithium salt.