Silantes offers a currently unrivaled product line for isotope labelled RNA and DNA:
With several years of experience, we are a reliable source for both, isotope-labelled RNA and DNA building blocks (NTPs and phosphoramidites), as well as a range of services for oligonucleotide synthesis using isotope-labelled building blocks.
This allows us to offer unmatched services and prices for stable isotope-labelled oligonucleotide synthesis and reagents.
Dependent on isotope labelling specification and sequence length, Silantes has developed different RNA synthesis strategies. For synthesis of uniformly stable isotope-labelled (2H, 13C, 15N, 18O, 19F) RNA oligonucleotides, Silantes uses the T7 RNA polymerase system for in vitro transcription.
Silantes has developed a cost-effective technology for synthesizing stable isotope-labelled DNA oligonucleotides. The enzymatic method differs from the conventional solid-phase synthesis and allows a more efficient conversion of isotope-labelled dNTPs into oligonucleotides, either isotope-labelling the entire oligonucleotide or all positions of a particular base.
For site-specific incorporation of stable isotope-labelled (2H, 13C, 15N, 18O, 19F) nucleotides into RNA or DNA sequences solid-phase chemical synthesis is the method of choice. For this purpose nucleoside phosphoramidites are used during the respective cycle of oligonucleotide synthesis.