SILAC RPMI Lysine(8) Arginine(10) Kit

SILAC RPMI Lysine(8) Arginine(10) Kit

Product no.: 284986444

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Description: SILAC RPMI Lysine(8) Arginine(10) Kit

Content of the Kit:

- 2x500mL RPMI 1640 (Roswell Park Memorial Institute) without Glutamine, Arginine Lysine

- 2x5mL Glutamine 200mM, (5mL Glutamine in 500mL DMEM to get a final concentration of 2mM)

- 2x50mL FBS (Fetal Bovine Serum, dialyzed and sterile)

- 1x500µL Lysine (13C6, 15N2 labeled) in solution (c=0,274mol/L)

- 1x500µL Lysine (unlabeled) in solution (c=0,274mol/L)

- 1x500µL Arginine (13C6, 15N4 labeled) in solution (c=1,15mol/L)

- 1x500µL Arginine (unlabeled) in solution (c=1,15mol/L)

The procedure in short:

Cells are grown in culture media that are identical with exception of amino acids. The “light” culture contains all the amino acids in unlabelled form and the “heavy” culture contains one or two amino acids in labelled form. The labelled amino acids are usually lysine labelled with 2H (D4), 13C or 13C15N and/or arginine labelled with 13C or 13C15N. The labelled amino acids increase the molecular weight allowing identification and quantification of the proteins/peptides stemming from culture light and heavy, respectively.

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